Dr Dishant Sharma

Dishant Sharma

Post doctoral Associate

Translational and Clinical Research Institute.
Newcastle University
LocationUnited Kingdom


Research Interests

Joubert syndrome, Nephronophthisis, Kidney diseases, Anti sense oligonucleotide, Gene Therapy

He is working on a project, “Use of antisense oligonucleotides as personalised therapies for kidney disease”. This is related to Renal ciliopathies: disease mechanisms and therapeutics. Nephronophthisis (NPHP), an autosomal recessive primary ciliopathy, is the most common cause of end-stage renal disease in children and young adults. Our research has revealed a critical role for hedgehog signalling in NPHP such that chemical agonists of smoothened can ameliorate disease phenotypes [Hynes et al.2014. PNAS 111:9893-8; Srivastava et al. 2017. Hum Mol Genet 26:4657-67]. Subsequently, we have demonstrated the therapeutic potential of exon-skipping antisense oligonucleotides (ASO) in patient cells and mouse models in vivo [Ramsbottom et al. 2018. PNAS 115: 12489-94; Molinari et al. 2019. Sci Rep 9:10828]. We now wish to exploit a novel murine model of NPHP and use ASOs to determine the exact therapeutic benefit and tissue delivery and assess off-target effects as a prelude to first-in-man studies.